Ultrastructural cytochemistry of the aldehyde-sensitive enzymes. Localization of glucose 6-phosphatase activity in mouse hepatocytes.

Title ULTRASTRUCTURAL CYTOCHEMISTRY OF THE ALDEHYDE-SENSITIVE ENZYMES : LOCALIZATION OF GLUCOSE 6-PHOSPHATASE ACTIVITY IN MOUSE HEPATOCYTES

Simultaneous demonstration of acid phosphatase and glucose-6-phosphate dehydrogenase in mouse hepatocytes. A novel electron-microscopic dual staining enzyme-cytochemistry.

Acid phosphatase (ACPase) and glucose-6-phosphate dehydrogenase (G6PD) play important roles in cell biology/disease pathophysiology in various organs including the liver. The purpose of the present report is to introduce a new enzymecytochemical method to simultaneously demonstrate the subcellular localization of ACPase and G6PD within the same hepatocyte in the mouse liver. The ultrastructural...

Localization and Activity of Mouse Endometrial Alkaline Phosphatase after Hyperstimulation and Progesterone Injection at the Implantation Time

The activity of mouse endometrial alkaline phosphatase after hyperstimulation and progesterone injection at the implantation time Alkaline phosphatase (ALP) of endometrium may play a critical function in the development and implantation of embryo. The aim of this study was to determine the localization of endometrial ALP activity after hyperstimulation and progesterone injection. Thirty adult f...

Demonstration of glucose-6-phosphate dehydrogenase in rat Kupffer cells by a newly-developed ultrastructural enzyme-cytochemistry.

Although various tissue macrophages possess high glucose-6-phosphate dehydrogenase (G6PD) activity, which is reported to be closely associated with their phagocytotic/bactericidal function, the fine subcellular localization of this enzyme in liver resident macrophages (Kupffer cells) has not been determined. We have investigated the subcellular localization of G6PD in Kupffer cells in rat liver...

O-10: A Marked Animal-Vegetal Polarity in The Localization of Na+,K+-ATPase Activity and Its Down-Regulation Following Progesterone-Induced Maturation

Background: Polarized cells are key to the process of differentiation. Xenopus oocyte is a polarized cell that has complete blue-print to differentiate 3 germ layers following fertilization, as key determinant molecules (Proteins and RNAs) are asymmetrically localized. The objective of this work was to localize Na+, K+-ATPase activity along animal-vegetal axis of polarized Xenopus oocyte and fo...